A Simple Approach for Obtaining High Resolution, High Sensitivity 1H NMR Metabolite Spectra of Biofluids with Limited Mass Supply

A simple approach is reported that yields high resolution, high sensitivity H NMR spectra of intact biofluids with limited mass supply. This is achieved by spinning a capillary sample tube containing a biofluid at the magic angle and at a spinning rate of about 80Hz. A 2D pulse sequence called H PASS is used to produce a high-resolution H NMR spectrum that is free from magnetic susceptibility induced line broadening. Using this new approach a high resolution H NMR spectrum of biofluids with a volume less than 1.0 l μ can be achieved at a magnetic field strength as low as 7.05T. Furthermore, the methodology facilitates easy sample handling and is suitable for high throughput investigations. In this paper first results are shown obtained at a magnetic field of 7.05T on urine samples collected from mice using a modified commercial NMR probe.